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Nevertheless, the assay didn't create trustworthy information in a number of biological examples. Therefore, we applied enzyme kinetics analysis from the fluorescent dNTP incorporation curves and found that the Taq polymerase shows a dNTP independent exonuclease task that decouples signal generation from dNTP incorporation. Also, we found that both polymerization and exonuclease tasks tend to be unpredictably inhibited because of the test matrix. To solve these pr
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