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82, p 0.01). In the vaginal tissue, a negative correlation was found for zinc and copper levels with the age of women (r = - 0.27, p = 0.04 and r = - 0.56, p 0.01). Multiple linear regression model (age, menopausal status, vaginal delivery and copper/zinc blood levels) showed that only age remained a significant predictor for zinc and copper vaginal tissues levels (p = 0.03, 95% CI - 2.28 to - 0.06; p = 0.004, 95% CI - 1.76 to - 0.34). Zinc and copper levels in the vaginal tissue decline with age. Out of the examined varia