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While earlier studies have recognized a quantity of interneurons and circuits that regulate muscle contraction in numerous mannequin methods, neural circuit mechanisms underlying the timing of muscle rest are less well explored6,7,eight. Here, we revealed crucial roles performed by Canon neurons within the regulation of muscle rest throughout backward locomotion of Drosophila larvae. The following evidence factors to the function of Canon neurons in muscle rest, not contraction. First, Canon neurons are activated much later than the downstream MNs. Additionally,
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