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V.Lotus leaf polysaccharides were removed by enzyme-assisted extraction utilizing α-amylase (LLEP-A), cellulose (LLEP-C), pectinase (LLEP-P) or protease (LLEP-PR). Their physicochemical properties and immunostimulatory activities had been in contrast to those of hot-water extracted polysaccharides (LLWP). HPAEC-PDA and HPSEC-RI profiles indicated that variants in their molecular body weight habits and chemical compositions. Additionally, their particular results on proliferation, phagocytic activity,