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The phytostabilization of mine tailings requires a previous assessment of the effects of soil amendments on metal mobility. The goal of this work was to evaluate the response of metal availability (both labile and potentially available pools) to the addition of two organic amendments (a municipal waste biosolid and a tree biochar), separately and in combination, in a mine tailings substrate. For this purpose, a comprehensive comparison among several single extraction procedures and a sequential extraction procedure was performed. The ef