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We succeeded in enhancing CTL activity by the CWS-NPs, and the findings reported herein should provide important information regarding target cells for the development of CWS-NP. We conducted a stability study of biodegradable and amphiphilic nanoparticles (NPs) consisting of phenylalanine attached poly(γ-glutamic acid) (γ-PGA-Phe) for drug delivery to find the optimal formulation, and define the optimal storage conditions using novel quantitative analytical methods. The stability of NP suspension and lyophilized NP powder manufactured by