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Further, the optimized 0.3 mol% V-doped g-C3N4 sample showed an antibacterial activity higher than that of the undoped photocatalyst.To investigate the effect of sludge ratio on the hybrid CANON system, autotrophic nitrogen removal sludge was inoculated with different granule/floc ratios to initiate the CANON system, and maintained the sludge ratio during the operation process. The start-up performances were compared, and the distribution characteristics of functional bacteria were investigated. The results show that the Equivalent syste