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Molecular markers are often more effective and precise in describing the hereditary functions for the recognition and purity evaluation of cultivars. In today's research, we assessed the distinctness and uniformity of five soybean cultivars using both single nucleotide polymorphism (SNP) markers manufactured by specific-locus increased fragment sequencing (SLAF-seq) technology, and easy sequence perform (SSR) markers. The phylogenetic tree and main element analysis (PCA) from both the