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d ≥ 65 years old) and males were relatively more sensitive to PM2.5, and were affected right away after the PM2.5 concentration went up.In this research, several biochemical variations in plant of Lemna minor L. were investigated to reflect Ag+ toxicity. Lemna minor L. changed colorless AgNO3 to colloidal brown at doses equal to and greater than 1 mg L-1. Optical and fluorescence microscopy revealed the presence of bright spots in roots of tested plant related to Ag/Ag2O-NPs. Photosynthetic pigment contents of Lemna minor L. declined upo