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Malignant glioma is a fatal brain cancer. Accumulated evidence has demonstrated that exosomes can cross the blood-brain barrier (BB, suggesting their potential use as drug delivery vehicles to glioma. Therefore, various loading methods of anticancer agents into exosomes have been developed. However, the loading efficiency of anticancer drugs, such as doxorubicin (DOX) and paclitaxel (PTX), into exosomes is relatively low, thus challenging to improve the drug delivery efficiency to glioma cells (GMs) via exosomes. To improve the loadi