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Whereas the adverse effects of vanadium released from smelting activities on soil microbial ecology have been widely recognized, little is known about spatiotemporal vanadium distribution and microbial community dynamics in typical contaminated sites. This study describes vanadium contents associated with health risk and microbial responses in both topsoil and subsoil during four consecutive seasons around an ongoing-production smelter in Panzhihua, China. Higher levels of vanadium concentration exceeding soil background value in China