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Diamond-like carbon (DLC) and titanium-doped DLC coatings were prepared by hybrid PECVD/direct current magnetron sputtering (DCMS). In this study, we show that the operating conditions of titanium-doped DLC coatings used for implants in surgical devices significantly modify their surface properties and consequently their interaction with cells. The coatings showed uniform distribution on the substrate and their biocompatibility was tested by way of rat calvaria osteoblasts. Doping DLC with Ti changed the roughness and wettability of the