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The absence or presence of buprenorphine in urine samples was evaluated by a validated gas chromatography mass spectrometry method. In order to determine the concentration of steviol glucuronide in urine samples, a liquid chromatography-tandem mass spectrometry method has been developed and fully validated according to the respective guidelines of the German Society of the Toxicological and Forensic Chemistry. Cross-reactivity of steviol glucuronide in the CEDIA® Buprenorphine II immunoassay was observed at concentrations above 15000 µ