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NS1-specific Western blot analysis additionally detected anti-NS1 antibodies in sera of humans who obtained numerous amounts of either of these two vaccines; however, many vaccinees which received ≤3 doses were negative for NS1-specific antibodies. The share of NS1-specific antibodies to defense against TBE had been shown by immunization of mice with purified NS1 antigen, which resulted in an important (p less then 0.01) prolongation of the mean survival ti
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