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Acinetobacter baumannii has traditionally been considered an opportunistic pathogen with low virulence. In this study, we characterized the carbapenem-resistant hypervirulent A. baumannii (CR-hvA stains isolated from our hospital in mid-south region of China. Blood samples collected between January 2017 and May 2019 were used for virulence experiments and biofilm assays of individual carbapenem-resistant A. baumannii (CR-A strains, performed using a Galleria mellonella infection model and crystal violet staining method, respectively. CR-AB isolates that