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In the present research, 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide indicated that miR-140 inhibited the viability of hPDLFs cells. Additionally, we provided research that miR-140 inhibited alkaline phosphatase (ALP) task, Alizarin Red S (ARS) task plus the mRNA phrase of osteogenesis associated genes, including ALP, runt-related transcription factor 2, collagen 1, and osteocalcin. Besides, double-luciferase reporter result shown that Ras homolog gene family members, member A (R