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So as to additional validate the function of mmi-DIGIRR and its own expression levels in various cells of fish, qRT-PCR was conducted. The outcomes showed DIGIRR features significant appearance levels in liver, skin and muscle tissue while phrase amounts in heart tend to be reasonable. The LPS-induced NF-κB activation was inhibited by overexpression of DIGIRR considerably. To conclude, the evolution and function of mmi-DIGIRR had been comprehensively analyzed in this