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Researches of NO production in GS cells after miR-29b transfection revealed that miR-29b overexpression affected NO production through the downregulation of IFNγ expression. Taken collectively, our results declare that miR-29b may directly target E. coioides IFNγs and modulate IFNγ-mediated natural immune answers by controlling E. coioides IFNγ production.Spermatogenesis is a complex and fancy differentiation procedure and is critical for male fertility. The hypothalamic-pituitary-gonadal