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36%, P= .02; EFS 35% vs. 0%, P= .03). Significant prognostic factors for EFS on multivariate analysis were the achievement of molecular remission (50 copies of WT1) at any time during treatment (hazard ratio [HR] 0.47, P= .04) and increased WT1 expression after 2 cycles of chemotherapy (HR 2.0, P= .03). Increased WT1 expression after 2 cycles of chemotherapy is a negative prognostic factor for survival. WT1 remains a valuable molecular marker in AML without any leukemia-specific mutation, especially if next generation sequencing an