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Nitrate (NO3) is the most common chemical contaminant in the world's ground water aquifer. Oxidative stress has been proposed as a possible mechanism involved in NO3 toxicity on non-target organism. The current study aimed to elucidate the potential protective effect of (pumpkin seed oil, PSO) against hepatotoxicity induced by sodium nitrate. Wistar rats were exposed either to NaNO (200 mg/kg bw) in drinking water in drinking water, or to 4ml PSO/kg bw by gavage or to their combination. Oxidative stress parameters, biochemical biomarke