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In this research, a novel formula of niosomal Ginsenoside Rh2 was ready with the thin film hydration method. The niosomal formulation included Span 60 and cholesterol levels, and cationic lipid DOTAP had been assessed by identifying particle dimensions distribution, encapsulation effectiveness, the polydispersity list (PDI), and surface morphology. The cytotoxic outcomes of no-cost Ginsenoside Rh2 and Ginsenoside Rh2-loaded niosomes had been determined using the MTT method in the PC3