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IJCEP Copyright © 2020.Precision medicine requires precise multi-gene clinical diagnostics. In present medical training, the minimum self-confidence threshold for variant calling of targeted next-generation sequencing (NGS) on medical specimens is scheduled to 2%-5%. Nevertheless, few research reports have already been performed to identify many actionable alternatives utilizing capture-based ultra-deep targeted sequencing, which includes limitation of recognition (LOD) of 1%. The AmoyDx® Essential NGS panel for capture-