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In the current study, improved exposure control and measurements were applied for the aquatic toxicity testing of a highly hydrophobic organic compound. The aim was to reliably determine the ecotoxicity of the model compound dodecylbenzene (DDB, Log KOW = 8.65) by applying passive dosing for aquatic toxicity testing exactly at the solubility limit. Methodologically, silicone O-rings were saturated by immersion in pure liquid DDB (i.e., "loading by swelling" and then used as passive dosing donors. Daphnia immobilization and fish embryo