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By means of ex vivo tissue deuterium-labeling using the stable isotope-labeled precursor [6,6,6-2H3]-(±)-mevalonolactone and microvinification experiments, we were able to show for the first time that the three sesquiterpene hydrocarbons, guaiazulene, δ-selinene, and selina-3,7(11)-diene, in Lemberger red wines do not originate from acid-catalyzed cyclization of yeast-derived farnesol and nerolidol. The three aforementioned sesquiterpene hydrocarbons could be unambiguously identified as grape-derived secondary metabolites and can theref