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Conclusion MDS 3.0 depression and behavioral symptoms had high overall and negative agreement, but low positive agreement with clinician diagnoses. MDS 3.0 data may be useful in ruling out depression and behavioral symptoms. Confirmation of the findings in a representative sample of nursing homes is warranted.Diosgenin (DI) and trigonelline (TRG) are the main bioactive compounds of fenugreek seed. A micro-extraction method using magnetic nanocomposites coupled with ion-pairing reversed phase HPLC (RP-HPLC) method was used to determine the analytes in rabbit