https://www.selleckchem.com/pr....oducts/simufilam.htm
The designed, NisC-cyclizable high-affinity ligands can be formed via mutant NisB-catalyzed dehydration, but less effectively via wild type NisB activity. In Lactococcus lactis, a cell surface display precursor was designed, comprising DSHPQFC. The preceding Asp residue of the serine in this sequence disfavors its dehydration by wild type NisB. The cell surface display vector was co-expressed with a mutant NisB library and NisTC. Subsequently, mutant NisB-containing bacteria that display cyclized strep-ligands on the cell surface were
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