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Moreover, miR-495 upregulation inhibited PI3K/AKT signaling pathway through downregulating PKIB. To sum up, this study reveals that miR-495 upregulation can reduce visceral sensitivity in IBS-D via inhibition of PI3K/AKT signaling pathway by targeting PKIB. It suggests that miR-495 presents a potential target for IBS-D therapy. © 2020 International Union of Biochemistry and Molecular Biology.BACKGROUND Emerging findings from neuroimaging studies investigating brain activity associated with dietary behavior are illuminating the interaction