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This study presents predictive modelling with uncertainty analysis, optimization and techno-economic feasibility of Bio-catalyzed Biodiesel Production from Azidirica Indica Oil (BCBPAIO). Central Composite Design (CCD) predictive model and optimum conditions for BCBPAIO were developed in Design Expert software. The model uncertainty analysis was performed using Monte Carlo simulation. The BCBPAIO simulation and economic analysis were conducted in ASPEN Batch Process Developer V10. The correlation coefficient (R2) and ad