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In summary, the developed method proved its ability in analyzing trace alkaloids in complicated biological samples. © 2020 John Wiley Sons, Ltd.OBJECTIVES To evaluate the ultrasound (US) findings of gastrointestinal anisakiasis and the utility of US in its early diagnosis. METHODS We retrospectively assessed the imaging findings and clinical data of 21 patients with gastrointestinal anisakiasis. Diagnosis was confirmed by a positive antigen (n = 16), endoscopy (n = 2), or a compatible clinical presentation, physical examination,