https://bapta-am0.com/while-li....nk-is-equal-to-causa
We stated in Escherichia coli Cdc8-tropomyosin bearing a phosphate team on Serine-125 (Cdc8 PS125), making use of an orthogonal tRNA-tRNA synthetase pair that straight incorporates phosphoserine into proteins as a result to a UAG codon when you look at the corresponding mRNA. We show utilizing complete internal reflection (TIRF) microscopy that, whereas E.coli produced Cdc8 PS125 does not bind actin filaments, Cdc8 PS125 incubated with lambda phosphatase binds actin filaments. This work right shows that a
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