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In this work, we present antiproliferative activity against individual cancer cells regarding the dinuclear CIII) buildings bearing the quinizarin ligand and tris(2-aminoethyl)amine (tren, ingredient 1) or tris(2-pyridylmethyl)amine (tpa, ingredient 2) co-ligands. To contribute the comprehension components of biological action of those compounds, their connection with DNA within the cells, DNA binding in cell-free news, and DNA cleavage capability had been examined in detail. The result