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Western blotting was utilized to detect FADS2 and CXCL15 protein appearance. Lactoferrin therapy at 10-500 μg/ml notably decreased the lipid content, as uncovered because of the oil red O and Nile purple staining. Moreover it attenuated the increase of mRNA expression of SREBP-1, FAS, SCD-1, FADS2, and IL-8 in insulin-treated SZ95 cells. Moreover, lactoferrin treatment in the amounts of 1-50 mg/mouse dramatically reduced the irritation and lipid manufacturing into the mouse model of pimples. Ad