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Exposure to prenatal sex steroids as indicated by the ratio of the second to fourth digit length (2D4D) has been linked to the risk of onset of cancer, while sex steroids may expand the gender disparity in bladder cancer (BC) morbidity. To explore the association between 2D4D ratio and BC risk. 307 bladder cancer patients and 321 cancer-free individuals. Relationships between 2D4D and incidence of bladder cancer. For males, a lower 2D4D ratio of both hands was obtained in the BC group, relative to the control group (left hand 0.940±0.0