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Global myocardial perfusion for sequential multi-slice experiments had been 1.64 ± 0.47, 1.34 ± 0.29, and 1.88 ± 0.58 for basal, mid, and apical SAX slices during diastole and was 1.61 ± 0.35, and 1.66 ± 0.49 for middle and apical slice during systole. These values tend to be similar to published ASL-CMR and positron emission tomography researches. SUMMARY SS-EPI is a promising alternative to bSSFP imaging for ASL-CMR and may possibly enhance the spatial protection of ASL-CMR by 3-fold during diastole and