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The mathematically validated ideal conditions of 45 °C and pH 7 had a statistical influence on the immobilization yield (IY) of papain in OxBC (52.9%). These ideal conditions were additionally used for papain immobilization in BC (unoxidized). The IY of 9.1per cent ended up being less than compared to OxBC. OxBC-Papain and BC-Papain were investigated using thermal analysis, confocal microscopy, and diffusion evaluation. The OxBC help exhibited a more interactive substance framework compa