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In this report, a sensitive fluorescence sensor for OPP recognition ended up being constructed in line with the alkaline phosphatase (ALP) -triggered in situ effect. In this process, ALP catalyses the dephosphorylation associated with substrate l-ascorbic acid 2-phosphate sesquimagnesium sodium hydrate (AAP) to generate l-ascorbic acid (AA). AA immediately combines with o-phenylenediamine (OPD) to form 3-(1,2-dihydroxyethyl)furo[3,4-b]quinoxalin-1(3H)-one (DFQ),