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© 2020 Wiley-VCH Verlag GmbH Co. KGaA, Weinheim.Methane emissions from peat bogs are mitigated by methanotrophs, which are now living in symbiosis with peat moss (e.g. Sphagnum). Right here, we investigate the impact of temperature and resultant changes in methane fluxes on Sphagnum and methanotroph-related biomarkers, assessing their potential as proxies in ancient bogs. A pulse-chase test making use of 13 C-labelled methane in the field clearly r