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To investigate the significance of multi-gene assay in papillary thyroid carcinoma (PTC) patients in clinical practice. From April to December 2019, medical records of 68 patients with PTC after the initial surgery were retrospectively collected and analyzed in terms of the relations between gene mutations and clinicopathological characteristics. RET/PTC rearrangement was not detected in BRAF V600E mutation patients (P0.001). Besides, compared with wild-type patients, BRAF V600E mutation was associated with significantly older