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Acanthopanax trifoliatus has been used as both traditional plant food and medicinal plant in Thailand. This study aimed to evaluate proximate, vitamin, and mineral compositions of A. trifoliatus leaf samples together with antioxidant and anticancer activities of ethanolic leaf extract of A. trifoliatus. For leaf samples, quantitative determination of proximate composition was evaluated comprising moisture, crude protein, total fat, total carbohydrate, dietary fiber, ash, as well as energy. Quantitative determination of vitamin and mine