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A berberine/hydroxylpropyl-β-cyclodextrin (HP-β-CD) inclusion complex was first willing to increase the solubility of berberine and packed into a thermoresponsive hydrogel system of poloxamers. A radioactive tracer of 125I-labeled berberine had been utilized to investigate brain targeting. Liquid chromatography along with tandem size spectrometry (LC-MS/MS) was performed to review t