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Of the techniques readily available for extracting complete RNA from Drosophila, monophasic lysis reagents (such TRIzol) yield consistent and reliable outcomes and so are dramatically cheaper than various other kit-based approaches for RNA isolation. In this protocol, up to 50 mg of Drosophila tissue is homogenized in a microcentrifuge tube utilizing a plastic pestle when you look at the presence associated with the monophasic lysis reagent. For larger amounts of starting product, homogenization can be perform
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