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Our results support the utility of FPIA for the detection of anti-IAV antibodies, especially the anti-H5 antibody.Welders have an increased risk for cardiovascular disease (CVD) following exposure to welding fumes. The underlying mechanisms are largely unknown; however, oxidative stress, systemic inflammation, and endothelial dysfunction have been suggested as contributing factors to particle-induced CVD. We investigated effects of mild steel welding fume (MSWF) on three target cell types macrophages, pulmonary epithelial, and vascular en