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The average concordance between SPM hypometabolism t-maps in the three patient cohorts, as obtained with the new datasets and compared to the HSR-HC standard reference dataset, was 0.87 for the AIMN-HC dataset and 0.83 for the ADNI-HC dataset. Pattern expression analysis revealed high overall accuracy ( 80%) of the SPM t-map classification according to different statistical thresholds and sample sizes. The applied procedures ensure validity of these HC datasets for the single-subject estimation of brain metabolism using voxel-wise