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Background Astrocytes are the main cellular constituent in the central nervous system. Astrocyte cultures from rodent brains are most commonly used in the experimental practice. However, important differences between rodent and human astrocytes exist. The aim of this study was to develop an improved protocol for routine preparation of primary astrocyte culture from adult human brain, obtained after trauma. New method Tissue obtained during neurotrauma operation was mechanically decomposed and centrifuged. The cell sediment was resuspende
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