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Therefore, this study sought the development of ferrocene (Fc)-labeled electrochemical biosensor for SNP detection. This proposed system involves the ligation of four short probes (e.g., A, B, A', and B', where B' is labeled with an Fc-tag) into the existence of target DNA via ligase chain reaction (LCR), leading to the forming of Fc-tagged duplex AB-A'B' in 2n. Consequently, immobilization of the Fc-tagged dupl