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The precipitation of Cu(II) by phosphate and the influence of dissolved organic matter (DOM) on the precipitation are essential for the fate of Cu(II) in aquatic environments. In this study, the influence of DOM on the reaction of phosphate with Cu(II) was investigated. Here, 51.61%, 29.75%, and 24.32% of the added Cu(II) (50 μM) precipitated without DOM and with the addition of fulvic acid (FA) and humic acid (HA), respectively, owing to the reaction with phosphate (50 μM). Excitation-emission matrix spectroscopy-parallel factor (PARAFAC) and two-dimension