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Iranian Registry of Clinical Trials IRCT20160427027647N2 . Registered on 14 May 2020. Iranian Registry of Clinical Trials IRCT20160427027647N2 . Registered on 14 May 2020. The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. This issue was addressed by rewiring glycerol metabolism for redistribution of t