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Background Our research is designed to explore the role of co-treatment of 5-FU and Pulsatilla decoction (PD) in the modulation of Immunogenic cell death (ICD) of Colorectal cancer (CRC). Materials and Methods Cell viability was evaluated by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide assays. Cell apoptosis was assessed using flow cytometry. The phosphorylation of STAT3 and expression of Mcl-1 and Bcl-xl were measured by western blot assays. The levels of ATP and HMGB1 in the supernatants of the culture medium were analyzed by