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To simulate hypoxic conditions in vitro, melanoma cells A2058, A375 and MeWo were cultured in a chamber with a gas blend of 5% CO2, 94% N2 and 1% O2. Among the list of cellular lines tested, MeWo cells presented better survival prices compared to A2058 and A375 cells. Co-cultures of L. lactis articulating GFP or mCherry and MeWo cells disclosed that L. lactis effortlessly express the transgenes under hypoxic conditions. Additionally, multispectral optoacoustic tomography (MSOT),
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