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Alteration gradients were identified in both studied regions based on the distribution of diatoms, with the first axis of distance-based redundancy analyses (dbRDA) being related to nutrient enrichment and organic loads. Threshold Indicator Taxa ANalysis (TITAN) performed on diatoms sampled along environmental (dbRDA axis 1 and phosphate) and biological gradients (as Ecological Quality Ratio scores of classifications), pointed to lower than current Good/Moderate boundaries for phosphate maximum values (e.g. 22.5 and 71.6 μg L-1 for Gali
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